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. 2018 Jun 4;45(5):565–579.e3. doi: 10.1016/j.devcel.2018.05.003

Figure 3.

Figure 3

N-cadherin Junction Formation Leads to Cell-Matrix Adhesion Disassembly near the Contact

(A) Outline and direction of migration of colliding cell pair, either in the absence or presence of N-cadherin (N-cadh) BA. Cells expressing membrane RFP (red) and GFP-FAK (green).

(B) Total area of GFP-FAK labeled CMAs at the contact during a collision. 0 min: first frame of contact, n = 3 collisions for both conditions.

(C) Control and N-cadherin BA treated cells expressing membrane RFP (gray) and GFP-FAK color coded for longevity.

(D) Longevity of CMAs near contact of cells expressing GFP-FAK. n = 30 for each condition.

(E) Total area of FAK labeled CMAs near the contact. n = 28 cells for both conditions.

(F and G) (F) Cells in clusters; zoom of region of contact shown by immunocytochemistry against p-paxillin in control cells (n = 171) cells expressing N-cadherin morpholino (n = 89) or treated with N-cadherin blocking antibody (n = 68). p-paxillin (green), phalloidin (red), Hoescht (blue). P-paxillin alone in left zoom colored according to fluorescence intensity (G). Total area of p-paxillin labeled CMAs at the contact. n = 68 cells.

Scale bars, 20 μm. Line graphs show median; errors, interquartile range. Bar graphs show mean; errors ± SEM. ∗∗∗p ≤ 0.001, ∗∗p ≤ 0.01. Mann-Whitney test. See also Figure S3.