Figure 5.

Src/FAK Inhibition Increases Cell-Matrix Adhesions near the Contact

(A and I) Immunocytochemistry against vinculin as indicated. Zoom of region of contact and free edge region shown in black boxes. Vinculin shown as green in merged image with Hoescht (blue) and cell outlines. Vinculin alone in zoom colored according to fluorescence intensity.

(B–E) Length and total area of vinculin labeled CMAs. Control, n = 33; PF-573228, n = 24; SU6656, n = 36 cells.

(F–H) Phalloidin staining and quantification as indicated. Arrows indicate phalloidin staining; dotted line indicates contact between adjacent cells.

(I) Immunocytochemistry against p-paxillin as indicated. Zoom of region of contact shown in black box. p-paxillin shown as green in merged image with phalloidin (red), Hoescht (blue), and cell outlines. P-paxillin alone in zoom colored according to fluorescence intensity.

(J) Total CMA area per cell near the contact. Control, n = 52; N-cadh BA, n = 50; N-cadh BA + Src Y527F, n = 49 cells.

Scale bars, 20 μm. Bar graphs show mean, error bars are ±SEM. ^∗∗∗p ≤ 0.001, ^∗∗p ≤ 0.01, ^∗p ≤ 0.05. All Kruskal-Wallis tests. See also Figure S4.