Skip to main content
. 2018 Jun 4;45(5):565–579.e3. doi: 10.1016/j.devcel.2018.05.003
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

anti-Phospho-paxillin pY118 ThermoFisher 44-722G
anti-vinculin Sigma-Aldrich V9131
anti-N-cadherin DSHB MNCD2
Anti-active Src clone 28 ThermoFisher AHO0051
anti-E-cadherin DSHB 5D3
N-cadherin blocking antibody – CDH2 ThermoFisher 13-2100

Chemicals, Peptides, and Recombinant Proteins

Src inhibitor – SU6656 Sigma-Aldrich S9692
FAK inhibitor – PF-573228 Sigma-Aldrich PZ0117
N-cadherin Fc chimera protein R&D systems 6626-NC
Fibronectin Sigma-Aldrich F1141
Phalloidin Rhodamine - TRITC Sigma-Aldrich P2141

Experimental Models: Organisms/Strains

Xenopus laevis European Xenopus Research Centre N/A

Oligonucleotides

N-cadherin morpholino antisense oligos
‘5′-GAAGGGCTCTTTCCGGCACATGGTG-3’
Gene Tools as described in (Theveneau et al., 2010) N/A

Recombinant DNA

Plasmid: FAK-GFP Gomez Lab at University of Wisconsin as described in (Myers and Gomez, 2011) N/A
Plasmid: constitutively active Src Y527F Yap Lab at University of Queensland, Australia N/A
Plasmid: P120-catenin GFP (Scarpa et al., 2015) N/A
Plasmid: N-cadherin - RFP (Scarpa et al., 2015) N/A
Plasmid: Src FRET biosensor Wang Lab at UC San Diego as described in (Wang et al., 2005) N/A

Software and Algorithms

Traction force microscopy analysis plugin This paper N/A
Focal Adhesion Analysis Server Developed by the Gomez Lab at Universtiy of North Carolina. (Berginski and Gomez, 2013) faas.bme.unc.edu
Image J NIH N/A
PRISM GraphPad N/A