REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
anti-Phospho-paxillin pY118 | ThermoFisher | 44-722G |
anti-vinculin | Sigma-Aldrich | V9131 |
anti-N-cadherin | DSHB | MNCD2 |
Anti-active Src clone 28 | ThermoFisher | AHO0051 |
anti-E-cadherin | DSHB | 5D3 |
N-cadherin blocking antibody – CDH2 | ThermoFisher | 13-2100 |
Chemicals, Peptides, and Recombinant Proteins | ||
Src inhibitor – SU6656 | Sigma-Aldrich | S9692 |
FAK inhibitor – PF-573228 | Sigma-Aldrich | PZ0117 |
N-cadherin Fc chimera protein | R&D systems | 6626-NC |
Fibronectin | Sigma-Aldrich | F1141 |
Phalloidin Rhodamine - TRITC | Sigma-Aldrich | P2141 |
Experimental Models: Organisms/Strains | ||
Xenopus laevis | European Xenopus Research Centre | N/A |
Oligonucleotides | ||
N-cadherin morpholino antisense oligos ‘5′-GAAGGGCTCTTTCCGGCACATGGTG-3’ |
Gene Tools as described in (Theveneau et al., 2010) | N/A |
Recombinant DNA | ||
Plasmid: FAK-GFP | Gomez Lab at University of Wisconsin as described in (Myers and Gomez, 2011) | N/A |
Plasmid: constitutively active Src Y527F | Yap Lab at University of Queensland, Australia | N/A |
Plasmid: P120-catenin GFP | (Scarpa et al., 2015) | N/A |
Plasmid: N-cadherin - RFP | (Scarpa et al., 2015) | N/A |
Plasmid: Src FRET biosensor | Wang Lab at UC San Diego as described in (Wang et al., 2005) | N/A |
Software and Algorithms | ||
Traction force microscopy analysis plugin | This paper | N/A |
Focal Adhesion Analysis Server | Developed by the Gomez Lab at Universtiy of North Carolina. (Berginski and Gomez, 2013) | faas.bme.unc.edu |
Image J | NIH | N/A |
PRISM | GraphPad | N/A |