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. 2018 Jun 1;22(6):879–892.e6. doi: 10.1016/j.stem.2018.05.003

Figure 2.

Figure 2

MEK1 Ablation Delays Return to Quiescence during Recovery from a Single 5-FU Injection and Promotes the Expression of Cell Cycle and OXPHOS Genes

(A–C) BM cellularity and HSCs per femur (A), HSC cell cycle distribution (B), and blood parameters (C) during the recovery from 5-FU injection.

(D and E) Heatmap (D) and scatterplots (E) of genes differentially expressed in F/F and cKO HSCs from untreated or 5-FU-treated mice (9 days prior isolation). Blue, increased expression in F/F HSCs; red, increased expression in cKO HSCs.

(F) qRT-PCR of selected cell cycle and OXPHOS genes in FAC-sorted HSCs. Data are normalized to expression levels in F/F HSCs from untreated mice (dotted line).

Error bars represent the SD of the mean. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 comparing CRE+ or F/F to cKO. White asterisks, comparison of 5-FU-treated versus untreated animals of the same genotype. See also Figure S2 and Table S1 for a complete gene list.