Reagent or Resource	Source	Identifier
Antibodies
α-mouse Gr-1-Biotin	eBioscience	clone RB6-8C5; RRID:AB_469757
α-mouse CD5-Biotin	eBioscience	clone 53-7.3; RRID:AB_466338
α-mouse CD2-Biotin	eBioscience	clone RPA-2.10; RRID:AB_466314
α-mouse CD3ε-Biotin	eBioscience	clone 145-2C11; RRID:AB_466320
α-mouse Ter119-Biotin	eBioscience	clone TER-119; RRID:AB_466796
α-mouse CD8a-Biotin	eBioscience	clone 53-6.7; RRID:AB_466345
α-mouse B220-Biotin	eBioscience	clone RA3-6B2; RRID:AB_466450
PerCP Streptavidin	BD Biosciences	Cat#554064; RRID:AB_2336918
α-mouse Sca1-PE	eBioscience	clone D7; RRID:AB_466085
α-mouse cKIT-APC-eFluor780	eBioscience	clone 2B8; RRID:AB_1272177
α-mouse CD150-APC	BioLegend	clone TC15-12F12.2; RRID:AB_493460
α-mouse CD48-PE-Cy7	BD Biosciences	clone HM48-1; RRID:AB_1727501
α-human CD34-APC	eBiosciences	clone 4H11; RRID:AB_2016604
α-mouse CD34-FITC	eBioscience	clone RAM34; RRID:AB_465020
α-human CD16/32-PE	eBioscience	clone 93; RRID:AB_465568
α-human CD38- PE-Cy7	BD Biosciences	clone HB7; RRID:AB_399969
α-mouse Gr-1-PE	eBioscience	clone RB6-8C5; RRID:AB_466046
α-mouse CD3ε-APC-eFluor 780	eBioscience	clone 145-2C11; RRID:AB_11149132
α-mouse B220-APC	eBioscience	clone RA3-6B2; RRID:AB_469394
α-mouse Mac1-PerCP-Cy5.5	eBioscience	clone M1/70; RRID:AB_953560
α-mouse CD45.1-FITC	eBioscience	clone A20; RRID:AB_465059
α-mouse CD45.2-AlexaFluor 700	eBioscience	clone 104; RRID:AB_657752
rabbit α-cleaved caspase 3	Cell Signaling Technology	clone 5A1E; RRID:AB_2070042
mouse α- α-MEK1	Cell Signaling Technology	clone 61B12; RRID:AB_10693788
rabbit α-pMEK1/2 S218/222	Cell Signaling Technology	Cat #9121; RRID:AB_331648
rabbit α-MEK1/2	Cell Signaling Technology	Cat #9122; RRID:AB_823567
rabbit α-pMEK1 T292	Upstate	Cat #07-852; RRID:AB_568845
rabbit α-MEK2	Cell Signaling Technology	clone 13E3; RRID:AB_2140641
rabbit α-ERK1/2	Cell Signaling Technology	Cat #9102; RRID:AB_330744
rabbit α-pERK1/2	Cell Signaling Technology	Cat #9101; RRID:AB_331646
rabbit α-AKT	Cell Signaling Technology	Cat #9272; RRID:AB_329827
rabbit α-pAKT S473	Cell Signaling Technology	Cat #9271; RRID:AB_329825
rabbit α-FOXO3a	Cell Signaling Technology	Cat #2497; RRID:AB_836876
rabbit α-pFOXO3a S294	Cell Signaling Technology	Cat #5538; RRID:AB_10696878
rabbit α-pFOXO3a S253	Cell Signaling Technology	Cat #9466; RRID:AB_2106674
rabbit α-mTOR	Cell Signaling Technology	Cat #2983; RRID:AB_2105622
rabbit α-p-mTOR S2448	Cell Signaling Technology	Cat #5536; RRID:AB_10691552
rabbit α-S6	Cell Signaling Technology	Cat #2217; RRID:AB_331355
rabbit α-pS6	Cell Signaling Technology	Cat #4858; RRID:AB_916156
rabbit α-PINK1	Abcam	Cat #ab23707; RRID:AB_447627
rabbit mAb IgG isotype control	Cell Signaling Technology	Cat #3900; RRID:AB_1550038
mouse mAb IgG isotype control	Cell Signaling Technology	Cat #5415; RRID:AB_10829607
goat α-rabbit IgG Alexa Fluor 488	ThermoFisher Scientific	Cat #R37116; RRID:AB_2556544
goat α-mouse IgG Alexa Fluor 488	ThermoFisher Scientific	Cat #A28175; RRID:AB_2536161
goat α-rabbit IgG Alexa Fluor 647	ThermoFisher Scientific	Cat #A27040; RRID:AB_2536101
goat α-mouse IgG Alexa Fluor 647	ThermoFisher Scientific	Cat #A28181; RRID:AB_2536165
mouse α-TOMM20	Abcam	Cat #ab56783; RRID:AB_945896
rabbit α-LAMP1	Abcam	Cat #ab24170; RRID:AB_775978
mouse α-p-Histone H2A.X (S139)	Millipore	clone JBW301; RRID:AB_309864
Chemicals, Peptides, and Recombinant Proteins
5-Fluorouracil	Sigma	Cat #F6627
N-acetyl-L-cysteine	Sigma	Cat #A7250
5-bromodeoxyuridine	Sigma	Cat #B5002
DMSO	Sigma	Cat #276855
U0126	Cell Signaling Technology	Cat #9903S
LY294002	Cell Signaling Technology	Cat #9901S
Metformin	Sigma	Cat #PHR1084
Rapamycin	Enzo Life Science	Cat #BML-A275-0005/0025
Polyethylene glycol (PEG) 400	Sigma	Cat #91893
TweenR 80	Sigma	Cat #P1754
CellROX Green Reagent	ThermoFisher Scientific	Cat #C10444
CellROX Deep Red Reagent	ThermoFisher Scientific	Cat #C10422
MitoTracker Green FM	ThermoFisher Scientific	Cat #M7514
MitoTracker Deep Red FM	ThermoFisher Scientific	Cat #M22426
TMRE	Sigma	Cat #87917
recombinant murine SCF	PeproTech	Cat #250-03
recombinant murine TPO	PeproTech	Cat #315-14
qRT-PCR primers	Sigma	Table S2
Critical Commercial Assays
FITC BrdU Flow Kit	BD Biosciences	Cat #559619
MethoCult M3434	StemCell Technologies	Cat #03434
MethoCult H4435 Enriched	StemCell Technologies	Cat #04435
Mouse LTC assay	StemCell Technologies	https://cdn.stemcell.com/media/files/manual/MA28417-Mouse_Long_Term_Culture_Initiating_Cell_Assays_Procedure.pdf
Human LTC assay	StemCell Technologies	https://cdn.stemcell.com/media/files/manual/MA28412-Human_Long_Term_Culture_Initiating_Cell_Assay.pdf
Fixable Viability Dye eFluor 450	eBioscience	Cat #65-0863-14
Fixation/Permeabilization Solution Kit	BD Biosciences	Cat #554714
FITC Mouse α-Ki-67 Set	BD Biosciences	Cat #556026
α-Biotin MicroBeads	Miltenyi Biotec	Cat #130-090-485
Deposited Data
RNA-seq data	this paper	GEO: GSE112842
Analysis of the F/F and MEK1 cKO hematopoietic compartments in different stress models	this paper; Mendeley data	https://doi.org/10.17632/7rdg6mjk5h.1
Validation of the antibodies used in the phosflow experiments	this paper; Mendeley data	https://doi.org/10.17632/7rdg6mjk5h.1
Experimental Models: Cell Lines
Human: CD34+ bone marrow progenitors	CellSystems	http://cellsystems.de/category/cd34-cells-1
Experimental Models: Organisms/Strains
Mouse: MAP2k1 (MEK1) F/F	Catalanotti et al., 2009	N/A
Mouse: VAV1-iCRE	de Boer et al., 2003	N/A
Mouse: C57/BL6 Ly5.1	Charles River Laboratories	N/A