Mild therapeutic hypothermia augmented expression of iASPP after stroke. (A) Representative protein bands of iASPP from the Western blot. Actin served as a loading control. Mice subjected to normothermic or mild hypothermic stroke were euthanized at 4, 12, 24 and 72 h later. (B) Densitometric quantification of iASPP from the Western blot. *p < 0.05, ***p < 0.001, vs sham group under normothermia; # p < 0.05, ### p < 0.001, vs sham group under mild therapeutic hypothermia; ♦♦ p < 0.01, ♦♦♦ p < 0.01, vs corresponding groups under normothermia, by two-way ANOVA and Sidak’s test, n = 4 per group. (C) Representative images showing localization of iASPP (red) in neurons (green) of the cortex at 24 h after stroke. Bar, 50 μm.