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. 2018 Jun 1;9(3):401–411. doi: 10.14336/AD.2017.0703

Figure 5.

Figure 5.

Effect of iASPPi and iASPPi plus hypothermia on the expression of iASPP and its targets in MCAO mice. (A) Representative protein in bands of iASPP, Puma, Bax and cleaved caspase-3 at 24 h after cerebral ischemia from the Western blot. Actin served as a loading control. (B) Densitometric quantification of iASPP, Puma, Bax and cleaved caspase-3 measured by Western blot. C-casp3, cleaved caspase-3, n = 4-6 per group. 37°C, normothermia. 33°C, mild therapeutic hypothermia. Small interfering RNA (siRNA) was intracerebroventricularly injected. Coni, control siRNA; iASPP siRNA (iASPPi). Data are expressed as mean ± SEM. N.S., not significant. *p < 0.05, **p < 0.01, ***p < 0.001, by one-way ANOVA and Tukey’s test.