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. 2018 Jun 1;9(3):412–425. doi: 10.14336/AD.2017.0926

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Copyright: © 2018 Zhang et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — Spatial distribution of CD45+ cells in brain parenchyma. Immunohistochemical staining reveals that major changes in CD45⁺ immunoreactivity (red) occur in the dentate gyrus (A, C, E) and hippocampal CA1 region (B, D, F). Cell nuclei are co-stained by DAPI (blue). In naïve (A and B) and sham operated (C and D) mice, strongly CD45 positive cells are scarce. On Day 3 after cardiac arrest and resuscitation, there is a significant increase in the number of CD45⁺ cells in dentate gyrus and CA1 region. The strongly positive immmunoreactivity in E-F as compared to that in A-D is likely related to the CD45^+hi subpopulation in Figure 5. (Scale bar = 15 μm).