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. 2018 Jun 5;8:8654. doi: 10.1038/s41598-018-26685-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Design of comparative PrP interactome study. (a) Models used in this study. (b) Flow-chart depicting workflow of quantitative interactome analyses. Note that for the NMuMG cell model, one additional step was inserted into the workplan, namely a 48-hour addition of Tgfb1 to the cell culture medium, which causes the cells to acquire mesenchymal morphology. (c) Similar SEQUEST X corr stratifications of the four cell type-specific PrP interactome datasets indicated comparable data quality.