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Primers for quantitative real-time PCR.
| Gene | Sequence 5′–3′ | |
|---|---|---|
| β-actin | Reverse | GCAAGCAGGAACGATGAG |
| Forward | CCATGCCAATGTTGTCTCTT | |
| IFNα | Reverse | GTGAGGAAATACTTCCAAAGAATCAC |
| Forward | TCTCATGATTTCTGCTCTGACAA | |
| IFNβ | Reverse | GCCGCATTGACCATGTATGAGA |
| Forward | GAGATCTTCAGTTTCGGAGGTAAC | |
| TLR3 | Reverse | TTTGCAAGAGGAATGTTTAAATCT |
| Forward | CACCTATCCGTTCTTTCTGAACTG | |
| TLR4 | Reverse | TCTTGGTGGAAGTTGAACG |
| Forward | GCCACACCGGGAATAA | |
| TLR7 | Reverse | GGAAATTGCCCTCGTTGTTA |
| Forward | CTTTTCACCCAGGCAGAATC |
