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. 2017 Dec 11;25(6):1131–1145. doi: 10.1038/s41418-017-0033-z

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© ADMC Associazione Differenziamento e Morte Cellulare 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Effects of Drp1 overexpression on ER–mitochondria contacts. Immunofluorescence against mitochondria (Tom20, cyan) and Drp1 (red) is shown in the corresponding panels. The green channel is the merge of several planes. Scale bar 20 µm. a Representative confocal pictures of HeLa cells expressing the SPLICS_S probe. b Quantification of SPLICS_S contacts by 3D rendering of complete z-stacks. Mean ± SEM: Ctrl 59 ± 3, n = 79 cells; Drp1 WT 70 ± 5, n = 32 cells; Drp1-K38A 89 ± 9, n = 28 cells. c Representative confocal pictures of HeLa cells expressing the SPLICS_L probe. d Quantification of SPLICS_L contacts by 3D rendering of complete z-stacks. Mean ± SEM: Ctrl 260 ± 14, n = 24 cells; Drp1 WT 198 ± 14, n = 24 cells. Data shown are the result of 3–4 independent experiments. **p ≤ 0.01.