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. 2018 Jun 5;8:8615. doi: 10.1038/s41598-018-27000-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing of NbHAOX8, NbGOX1 and NbGOX4 reduced resistance to TRV in N. benthamiana. (A) Phenotypes of the gene-silenced plants. Gene silencing analyses were performed using TRV-based vectors. Photographs were taken at 3 weeks post agro-infiltration. (B) Detection of transcripts of the TRV1 replicase and TRV2 2b genes in gene-silenced plants by qRT-PCR. (C) Evaluation of gene silencing efficiency. Gene expression was examined by qRT-PCR. Significant difference between expression values in gene-silenced plants and eGFP control plants is indicated as different lowercase letters (P < 0.05, DMRT).