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. 2018 Jun 5;8:8615. doi: 10.1038/s41598-018-27000-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing of NbHAOX8, NbGOX1 and NbGOX4 differently modulated H₂O₂ in N. benthamiana. (A) Detection of Xoo-elicited H₂O₂ in gene-silenced plants at 12 hpi by DAB staining. No HR occurred in these plants (upper panel). (B) Dynamics of H₂O₂ accumulation in response to flg22 (400 nM) elicitation in leaves of gene-silenced plants. Flg22-triggered H₂O₂ bursts were measured using luminol-based assay in leaf discs. Data are shown as relative luminal units (RLU) and represent the mean ± SE of three independent experiments. (C) Effect of silencing of NbHAOX and NbGOX genes on expression of RbohD and RbohF genes. Gene expression was analyzed by qRT-PCR. Data represent the mean ± SE of three independent experiments. Significant difference between expression values is indicated as lowercase letters (P < 0.05, DMRT).