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. 2018 Jun 5;8:8615. doi: 10.1038/s41598-018-27000-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing of NbHAOX8, NbGOX1 and NbGOX4 differently altered defense related genes and other member genes of NbGOX family in N. benthamiana. (A) Expression profiles of PR1, NPR1, PDF1.2, WRKY45 and WRKY62 genes in gene-silenced plants. (B) Mutual gene expression modulation of NbHAOX8, NbGOX1 and NbGOX4. Expression of the other two genes in single GOX gene-silenced plants was examined by qRT-PCR. Data represent the mean ± SE of three independent experiments. Significant difference between expression values is indicated as different lowercase letters (P < 0.05, DMRT).