Fig. 2.

FOXQ1 regulates cAMP/CREB1-dependent pigmentation. a NHM were treated with the indicated doses of Forskolin (FSK) for 5 h (left panel) or with 50 μM of FSK for indicated duration (right panel) followed by immunoblotting with the indicated antibodies. b NHM were transduced with control (CL) or FOXQ1 (F1, F2) shRNAs and treated with vehicle (DMSO) or the indicated doses of Forskolin (FSK) for 5 h followed by immunoblotting with the indicated antibodies. c Melanin content in cells described in b. d Representative images of ears from Foxq1^+/+ or Foxq1^–/– mice (n = 3) treated with either vehicle (DMSO, left ear) or vehicle containing 100μM Forskolin (FSK, right ear). e Pigmentation was quantified using a reflectance spectrometer and represented as fold change in the coefficient of absorption “mua”. f Representative images of H&E staining of mouse ear tissues shown in d. All data represent mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-tests. A p < 0.05 (*) was considered significant