Fig. 4.
BRAF-β-catenin-FOXQ1 axis controls MITF-dependent phenotypes. a NHM and Melan-a cells were transduced with indicated constructs (V = empty vector, B = BRAFV600E, F = FOXQ1) followed by immunoblotting with the indicated antibodies. b Cells were transduced with the indicated constructs and probed in Q-RT-PCR. (MITF/ACTB (Mitf/Actb) and FOXQ1/ACTB (Foxq1/Actb) signal ratios are shown. c Total melanin content in cells described in a. d NHMs transduced with the indicated constructs were probed in immunoblotting with the indicated antibodies (left panel) and in Q-RT-PCR (right panel). FOXQ1/ACTB signal ratios are shown. e Melan-a cells were transduced with the indicated constructs and assayed for the ability to form colonies in soft agar. Shown are average numbers of colonies per view field (n = 5). All data represent mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-tests. A p < 0.05 (*) was considered significant