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. 2018 Jun 5;9:2175. doi: 10.1038/s41467-018-04567-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — VentX-regulated TAMs modulate composition and function of TILs in tumor microenvironment. a, b Composition of CD4+CD25+ Tregs (a) and CD8+ TILs (b) in tumors after en bloc co-culture of tumor tissues with autologus TAMs transfected with GFP or GFP-VentX for 5 days. c CD8 TILs were isolated from tumor tissues after being incubated with autologus TAMs transfected with GFP or GFP-VentX for 5 days. CD8+TIL activation was measured by FACS analysis of IFNγ and granzyme B. Data shown are mean ± SD of three independent experiments and paired Student’s t-test was performed. *p < 0.05