Fig. 6.

VentX-regulated TAMs modulate tumorigenesis in vivo. a A NSG-PDX model of human colon cancers. Small pieces of colon cancer tissues were transplanted into subcutaneous space on the abdominal side of NSG mice and the growth of the tumors were observed for 8 weeks. Data shown were results of seven independent replicates of the experiments. b H&E and CK20 staining of tumors resected from three NSG-PDX mice. Scale bar: 50 μm. c Growth curve of tumors in NSG-PDX mice treated with TAMs transfected with GFP-VentX or control GFP. Results shown represent mean ± SD of three independently replicated experiments, n = 3, and paired Student’s t-test was performed, **P < 0.01. d Growth curve of tumors in NSG-PDX mice treated with M1-TAMs transfected with VentX-MO or control-MO. Results shown represent mean ± SD of seven independently replicated experiments, n = 7, and paired Student’s t-test was performed, **P < 0.01. Photos of excised tumors at the end of experiments were shown. Scale bar: 5 mm