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. 2018 Jan 8;25(6):1174–1188. doi: 10.1038/s41418-017-0037-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 2 — a Seminiferous tubules paraffin sections from Trf1^Flox/Flox and Stra8-Trf1^−/− testis were stained with PAS-hematoxylin. A type A spermatogonia, In intermediate spermatogonia, B type B spermatogonia, PL preleptotene spermatocytes, L leptotene spermatocytes, Z zygotene spermatocytes, P pachytene spermatocytes, aP apoptotic pachytene spermatocytes, M meiotic divisions, aM abnormal meiotic divisions, sS secondary spermatocytes, rSt round spermatids, S spermatids. b Spermatocyte stages in pre-metaphase in Trf1^Flox/Flox and Stra8-Trf1^−/− spermatocytes. Trf1^Flox/Flox and Stra8-Trf1^−/− chromosome spreads of spermatocytes were immunostained with antibodies against SYCP3 (green) and DAPI (blue). c Meiotic stage frequencies in Trf1^Flox/Flox and Stra8-Trf1^−/− testes. Lep (Leptotene): Trf1^Flox/Flox (gray bar, 12.07 ± 0.94%), Stra8-Trf1^−/− (white bar, 20.85 ± 1.30%). Zyg (Zygotene): Trf1^Flox/Flox (gray bar, 6.71 ± 1.34%), Stra8-Trf1^−/− (white bar, 23.63 ± 6.42%). Pac (Pachytene): Trf1^Flox/Flox (gray bar, 55.31 ± 2.50%), Stra8-Trf1^−/− (white bar, 47.60 ± 2.75%). Dip (Diplotene): Trf1^Flox/Flox (gray bar, 23.00 ± 2.71%), Stra8-Trf1^−/− (white bar, 7.00 ± 0.89%). Dia (Diakinesis): Trf1^Flox/Flox (gray bar, 2.88 ± 1.49%), Stra8-Trf1^−/− (white bar, 0.89 ± 0.52%). Data are presented as mean ± SEM. **P < 0.01, ***P < 0.001. d Representative TUNEL results in Trf1^Flox/Flox and Stra8-Trf1^−/− testes. Paraffin sections from Trf1^Flox/Flox and Stra8-Trf1^−/− testes were stained with TUNEL (green) and DAPI (blue) to determine apoptotic cells in stage IV and stage XII tubules together with pachytene and metaphase spermatocytes.