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. 2018 Jun 5;8:8604. doi: 10.1038/s41598-018-26955-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Size distributions and examples for the transcriptional overlaps. Panel a shows the comparison of size distributions of transcriptional overlaps of previously annotated transcripts (grey) versus previously annotated and novel transcripts with a certain TSS (black). Colour code: The red lines represent the mean values. Panel b Parallel; Panel c Divergent, and Panel d Convergent overlaps of previously annotated and novel transcripts. Reads from the Illumina and MinION cDNA sequencing were visualized using IGV, and their schematic representation. Grey arrow-rectangles represent previously annotated transcripts, while blue arrow-rectangles represent novel transcripts. The annotations were cut to fit the representation, indicated by a non-continuous annotation on one side.