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. 2018 Jun 5;8:8637. doi: 10.1038/s41598-018-26882-8

Figure 2.

Figure 2

NNMT expression in the livers of obese mice and the generation of NMMT transgenic mice. (A) NNMT mRNA expression, normalized to 36B4 levels, in the livers of genetically obese (db/db) mice (black bars) and their normal (db/+) littermates (white bars) (n = 4, 5 per group). (B) NNMT protein levels in the livers of obese (db/db) and lean (db/+) mice. (C) NNMT mRNA expression levels in WAT and livers of Tg mice (black bars) and WT littermates (white bars) were measured by real-time PCR (n = 5 per group). NNMT protein levels in livers of Tg mice and WT mice were determined by Western blot analysis. (D) Body weight and liver, epididymal, and inguinal fat weight/body weight ratios, after being fed an HFD for 3 months, in Tg and WT mice (n = 3 per group). (E) H-E staining (upper panel) and Masson trichrome staining (lower panel) of liver from Tg and WT mice fed an HFD for 3 months. Bars represent 1.0 mm. NAFLD activity score and Masson trichrome stained blue area (%) were assessed (n = 3 per group). **P < 0.01 versus WT mice. Full-length blots/gels are presented in Supplementary Fig. 6. Abbreviations: NNMT, nicotinamide N-methyltransferase; WAT, white adipose tissue; PCR, Polymerase chain reaction; Tg, transgenic; WT, wild type; HFD, high fat diet.