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. 2018 Jun 5;8:8640. doi: 10.1038/s41598-018-26856-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CCL5 increases the migration of HT29 and DLD-1 cells. (a) HT29 and DLD-1cells were treated with CCL5 and then assayed for cell migration as described in methods and materials. Representative images of migration are shown. (b) Quantification of the migration of HT29 and DLD-1 cells. (c) HT29 and DLD-1 cells were exposed to alcohol or pretreated with CCL5 antibody as described in methods and materials. HT-29 and DLD-1 cells were induced by alcohol and transfected with siCCL5. After that, images of cells migrating through the chambers were assayed. (d) The cells migrated through the chamber were quantified. Each data point was the mean ± SEM of three independent experiments and presented relative to the controls. **P < 0.01.