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. 2018 Jun 5;8:8588. doi: 10.1038/s41598-018-26901-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Wnt treatment on MSCs gene expression. MSCs were cultured to confluence on TCPS and treated with fresh media supplemented with 50 ng/mL recombinant human Wnt11 for 12 hours. RNA was extracted and gene expression analysis was conducted by RT-qPCR for runt-related transcription factor 2 (RUNX2), collagen type 1 (COL1A1), bone gla-protein (BGLAP), and alkaline phosphatase (ALPL A). Interaction of Wnt treatments on WNT gene expression was examined by RT-qPCR for WNT3A, WNT5A, and WNT11 (B) after treatment for 12 hours with 50 ng/mL of Wnt3a, Wnt5a, or Wnt11. ^*p < 0.05 vs control; ^#p < 0.05 Wnt3a treatment; ^%p < 0.05 vs Wnt5a treatment. RT-qPCR was conducted for WNT11 for MSCs cultured on TCPS or Ti surfaces for either 2, 4, or 6 days (C). ^*p < 0.05 vs TCPS; ^#p < 0.05 vs PT.