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. 2018 Jun 5;8:8588. doi: 10.1038/s41598-018-26901-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The role of Wnt11 in cell attachment and differentiation of MSCs on Ti substrates. WT and siWNT11 MSCs were cultured on Ti substrates and TCPS and cultured till confluence. Fresh media was incubated for 12 hours and RNA was extracted. Real-time qPCR was conducted for ITGA1 (A), ITGA2 (B), ITGA5 (C), ITGB1 (D), WNT5A (E), and RUNX2 (F). ^*p < 0.05 vs TCPS for each cell type; ^#p < 0.05 vs Control MSCs on each surface.