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. 2018 Jun 5;8:8588. doi: 10.1038/s41598-018-26901-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wnt11 is necessary for differentiation of MSCs on Ti substrates. WT and siWNT11 MSCs were cultured on TCPS, PT, SLA, and modSLA till confluence receiving media with or without 50 ng/mL of Wnt11. At confluence, cell number (A), alkaline phosphatase specific activity (B), and levels of secreted osteocalcin (C), osteoprotegerin (D), BMP2 (E), BMP4 (F), VEGF (G), and FGF2 (H) were determined. *p < 0.05 vs TCPS; ^#p < 0.05 vs WT MSCs on each surface; ^$p < 0.05 vs siWNT11 MSCs on each surface; ^%p < 0.05 vs WT + Wnt11 MSCs on each surface.