Fig. 5.

S183E breaks interactions within the core of casp-9. a Anion exchange chromatogram (bottom) and Coomassie-stained gel of peak fractions (top) for CT WT casp-9. CARD + Lg and Sm subunits co-elute and corresponding band intensities co-vary along the salt gradient during an anion exchange column, suggesting tight interaction of the subunits in the casp-9 core. b Anion exchange chromatogram (bottom) and Coomassie-stained gel of peak fractions (top) for CT S183E. The independent elution of the Sm subunit from the CARD + Lg indicates dissociation of core subunits of S183E