Table 1.

Catalytic parameters for casp-9 alanine variants using Ac-LEHD-afc as substrate.

Casp-9 variant	KM (µM)	kcat (s−1)	103×kcat/KM (µM−1s−1)
S99A	768 ± 100	0.64 ± 0.04	0.83
S195A	681 ± 71	1.22 ± 0.05	1.8
S99A/S195A	469 ± 41	1.03 ± 0.03	2.2
S99A/S183A	1203 ± 100	1.16 ± 0.05	0.96
S195A/S183A	1318 ± 107	1.17 ± 0.05	0.89
S99A/S183A/S195A	726 ± 71	0.20 ± 0.01	0.26

Note: Values are mean ( ± SEM) of three trials done on three separate days