Fig. 1.

Activated mTOR stimulates PGAM1 expression. qRT-PCR a and immunoblotting b of cell lysates extracted from WT, Tsc2^−/−, or Pten^−/− MEFs treated with or without 10 nM rapamycin (R) for 24 h. c Immunoblotting of lysates from kidney tumor and non-tumor kidney tissues of Tsc2^del3/+ mouse. d Left panel: Rat ELT3 cells treated with or without 10 nM rapamycin for 24 h were subjected to immunoblotting. Right panel: Immunoblotting of ELT3 cells with/without restoration of TSC2. e Protein lysates were extracted from human NSCLC tissues and adjacent tissues and then subjected to immunoblotting. Phosphorylation of S6 (pS6) is a marker of mTOR activity