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. 2018 Jun 5;8:8617. doi: 10.1038/s41598-018-26974-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of terrein on cellular c-di-GMP. (a and b) Cellular c-di-GMP levels in biofilm cells of PAO1 cultured with different terrein or furanone C-30 concentrations for 9 h. After the biofilms were dissociated from the wells by gentle sonication, cellular c-di-GMP was extracted from the biofilm cells, measured, and normalized to the total proteins. (c and d) Comparison of biofilm formation by the P. aeruginosa wspF mutant versus wild-type PA14 cells cultured with different terrein or furanone C-30 concentrations for 9 h. Three independent experiments were performed in triplicate, and the mean ± SD values are displayed in each bar. *P < 0.01; **P < 0.001; ***P < 0.0001 versus DMSO treatment.