Table 2.
| TMOD2 | ||||
| Anneal | ||||
| Exon | (° C) | Forward Primer (5'-3') | Reverse Primer (5'-3') | Product size (bp) |
| 1 | 54 | TTCAATACAGTAGGCTCCA | CGAGCTAAGGGATTTCTAC | 393 |
| 2 | 55 | GGTATCCTTCCTGCTTCC | GGCTTATTTCCCATGTCT | 413 |
| 3 | 54 | TCTGCTCAGTCTACTTACCC | CCATAATGCCTTGTGTCA | 322 |
| 4 | 54 | TTCAAGGGACAAATTAACA | AAAGCCCAAGTGATCCTA | 289 |
| 5 | 54 | AGTGTTATTGGAATCAGCAT | CTCCAACTCAGTGACTACAAA | 358 |
| *GTAAAACGACGGCCAGTTGCTGAAGGCTA | **GGAAACAGCTATGACCATGACTATAAT | |||
| 6 | 57 | GGTAATGA | TTTGCTCACTAACTGT | 390 |
| 7 | 54 | TTAATTTGGGAGTGAGGG | ACAGGAAATCCAGTACATCA | 356 |
| 8 | 54 | CCTGTGCAAGTAACTGTTTC | AGTGACATCAGAGGCCAG | 323 |
| 9 | 54 | ATGTTGTTTATCTGGAATTTA | CCCATGAGCAAATAAAAC | 307 |
| *indicates a M13 universal sequence attached | **indicates a M13 reverse sequence | |||
| at 5' end | attached at 5' end | |||