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. 2018 Jun 5;8:8627. doi: 10.1038/s41598-018-26763-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in Clostridia in cecal contents from ABX-treated chickens after co-housing with control chickens. Chickens were treated with ABX at hatching for 7 days and then co-housed with control chickens for 7 days. 16S rRNA sequencing was performed to determine the relative abundance of (A) Phylum, (B) Class, (C,D) Family, and (E,F) Genus in cecal contents at 1/4 (6 hours), 1, 3 and 5 days before co-housing (Before), and 1/4 (6 hours), 1, 3, and 5 days after co-housing (After). Data were obtained from more than four chickens in each group and presented as the mean ± SD. Significant differences between Con and ABX are shown with asterisks. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001.