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. 2018 Jun 5;8:8627. doi: 10.1038/s41598-018-26763-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in CD4⁺CD8⁻CD25⁺ and CD4⁺CD8⁺CD25⁺ T cells in chickens treated with acetate. SCFAs (acetate 50 mM, butyrate 30 mM, propionate 10 mM) and/or ABX in drinking water were administered to chickens at hatching for 7 days. The numbers of (A,C,E) CD4⁺CD8⁻CD25⁺ and (B,D,F) CD4⁺CD8⁺CD25⁺ T cells in cecal tonsils were calculated. (G) Subsets of CD4⁺ T cells, B cells (Bu-1⁺), and APCs (KUL01⁺, MHC class II (MHC2)⁺KUL01⁻Bu-1⁻) were sorted using an ARIA II FACS sorter. mRNA was extracted from each subset, and the expression level of GPR43 was determined by RT-qPCR. (A–G) Data were obtained from more than three chickens in each group and presented as the mean ± SD. Different characters indicate significant differences at P < 0.05. The figures are representative of three independent experiments.