Fig. 2.

USP10 is a transcriptional target of c-Myc. a Diagram of the USP10 promoter. There are five c-Myc binding elements, E-box sequences, upstream of the transcription start site of USP10. b A ChIP assay was performed using c-Myc antibodies in HFF and IMR90 cells stably expressing c-Myc. Relative enrichment was determined by quantifying PCR products obtained from three experiments. c 0.5 μg USP10 luciferase reporter constructs and 0.3–0.5 μg c-Myc-expressing plasmid were transfected into HFF cells as indicated. Luciferase activities of each reporter were analyzed by the luciferase assay. Cell lysates were immunoblotted with specific antibodies. This experiment was conducted in triplicate. d Luciferase activities of 0.5 μg USP10 luciferase reporter constructs, which have E-box sequence mutations of CAATTG, were analyzed using a luciferase assay. This experiment was conducted in triplicate