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. 2018 May 30;9:1160. doi: 10.3389/fmicb.2018.01160

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Copyright © 2018 Lai, Lin, Chen, Hsieh and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transposon disruption of three ESX-1-related genes significantly impairs sliding motility but not growth. (A) Transposon insertion sites within the ESX-1 locus. The three disrupted genes (gray) were espE (mmar_5439), espF (mmar_5440), and eccA1 (mmar_5443). The bold vertical lines indicate the transposon insertion sites. (B) Sliding motility of the three transposon mutants (8-C7, 22-B5, and 23-B1). Aliquots of 1 μL bacteria culture (OD₆₀₀ = 1) were added to 6-well sliding agar plates and cultured at 32°C for 1 week. (C) Bacteria were cultured in 7H9 medium supplemented with 10% oleic acid/albumin/dextrose/catalase (OADC), 0.5% glycerol, and 0.05% Tween-80. The growth of different strains was assessed based on OD_600nm. Here and below, all data are from three independent experiments and presented as means ± SDs with one-way ANOVA.