Figure 3.

Mg²⁺ elevation suppresses the expression of TNF-α, PEN2 and NCT in cultured neurons and glial cells. Human glioblastoma A172 (A), mouse astrocytes D1A (B) or microglia BV2 cells (C) were treated with MgT (50 μM) for 48 h; TNF-α mRNA and protein levels were determined by qRT-PCR and western blots. GAPDH and β-actin served as the internal control. In select experiments, human or mouse neuroblastoma SH-SY5Y (D) and n2a (E) cells were treated with MgT (50 μM) for 48 h. mRNA and protein levels of PEN2 and NCT were determined by qRT-PCR and western blots. GAPDH and β-actin served as the internal control. The data represent means ± SE of three independent experiments. *p < 0.05 and ***p < 0.001 compared with the vehicle-treated controls.