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Ryanodine binding. Effect of T2 on the density of ryanodine binding sites [Bmax, fmol/mg, (A)] and affinity for ryanodine [Kd, nM, (B)] in crude cardiac homogenates. Ryanodine binding was determined in ventricle homogenate after 50 min of perfusion. Histograms represent mean ± SEM derived from three hearts per group. Unpaired t-test yielded P = ns for the difference between groups.
