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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Semin Cell Dev Biol. 2017 Dec 6;81:46–53. doi: 10.1016/j.semcdb.2017.11.024

Figure 3. Defects in spermatid polarity following c-Yes knockdown by RNAi.

Figure 3

Testes from adult rats received a transfection mixture containing 100 siRNA duplexes specific to c-Yes on day 1 and day 3, i.e., 2 transfections for RNAi to knockdown c-Yes by >70% as described [27]. Control testes received the same amount of non-targeting negative control siRNA duplexes. On day 5, rats were euthanized and testes were removed to freeze in liquid nitrogen. Frozen sections were obtained and cell nuclei stained for DAPI (4′,6-diamidino-2-phenylindole). As noted herein, a knockdown of c-Yes, but not in control testes received the negative non-targeting control siRNA duplexes, caused extensive defects in spermatid polarity in which the head of spermatids no longer pointed towards the basement membrane, but deviating by 90° to 180° from the intended orientation (annotated by white arrowheads). Scale bar, 80 μm and 40 μm in the left and right panel.