Figure 2. Disparities in wVitA titers begin during oogenesis.
(A) Diagram of a Nasonia egg chamber. Large green circles represent nurse cell nuclei and small black circles represent Wolbachia. (B) Stage 3 egg chambers with host and Wolbachia DNA stained with SYTOX Green from wVitA-infected N. vitripennis 12.1. A = anterior, P = posterior, Scale bar = 15 μm. Examples of Wolbachia bacteria are labeled with a “W” and white arrows. (C) An embryo with host and Wolbachia DNA stained with HSP60 from wVitA-infected N. vitripennis 12.1. (D) Stage 3 egg chambers with host and Wolbachia DNA stained with SYTOX Green from uninfected N. vitripennis 12.1T. (E) Stage 3 egg chambers with host and Wolbachia DNA stained with SYTOX Green from wVitA-infected N. giraulti IntG. (F) An embryo with host and Wolbachia DNA stained with HSP60 from wVitA-infected N. giraulti IntG. All embryo and ovary images are representative of two and three independent experiments, respectively.
See also Figure S1