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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: J Immunol. 2018 Apr 25;200(12):4094–4101. doi: 10.4049/jimmunol.1701716

Figure. 3.

Figure. 3

Characterization of the C3 KO rats. A. Determination of serum C3 concentration. C3 levels in the sera from WT and C3 KO rats (n=5 in each group) were measured by ELISA following the manufacturer provided protocol; approximately 0.8 mg/ml C3 protein was detected in WT rats with no detectable C3 protein in the C3 KO rats. *p<0.05 B. C3 deposition assay. Serum (20%) from WT (dotted line) or C3 KO rats (solid line) were incubated with zymosan for complement activation. Then the deposited C3b/iC3b was assessed by staining the zymosan with a polyclonal anti-mouse C3 IgG (cross-react with rat C3) followed by flow cytometric analysis (shaded area, isotype control, ctrl).