Figure 1. Spontaneous lethal inflammation in mice with a Treg cell-specific deletion of talin.

(A and B) Morphology (A) or organs (B) (spleen, lymph nodes (aortic, brachial, mesenteric, inguinal), and thymi) of male Tln1^fl/fl or Tln1^fl/flFoxp3^Cre mice. (C) H&E stains of lung, liver, heart, and skin tissue from Tln1^fl/fl or Tln1^fl/flFoxp3^Cre mice. (D and E) Body weight from 4–10 weeks of age (D) and survival (E) of Tln1^fl/fl or Tln1^fl/flFoxp3^Cre mice; n=10. (F–H) Percentages of splenic CD4⁺ or CD8⁺ T cells (F) expressing CD44, CD62L (G), or Ki-67 (H) from Tln1^fl/fl or Tln1^fl/flFoxp3^Cre mice; displayed cells gated on CD4⁺ or CD8⁺ events; n=6. (I) IFNγ and TNFα expression by splenic CD4⁺ (left) and CD8⁺ (right) T cells from Tln1^fl/fl or Tln1^fl/flFoxp3^Cre mice; displayed cells gated on CD4⁺CD44^hi or CD8⁺CD44^hi events; n=6. Data are mean ± SEM and representative of at least 2 independent experiments. *, P < 0.05; **, P <0.01, *** P <0.005, unpaired Student’s t-test.