Figure 2. Talin deficiency decreases Treg cell suppressive capacity.

(A–B) Percentages (n=6) (A) and numbers (n=10) (B) of splenic CD4⁺ T cells from Tln1^fl/fl or Tln1^fl/flFoxp3^Cre mice expressing Foxp3. (C) Foxp3⁺ cells as a percentage of CD4⁺ T cells within tissues (blood, thymus, liver, lung and lymph node) derived from Tln1^fl/fl or Tln1^fl/flFoxp3^Cre mice; n=6. Mice were perfused prior to isolation of cells from liver and lung. (D) Mean fluorescence intensity of Foxp3 from talin-deficient and wild-type Treg cells; n=7. (E) Expression of putative suppressor molecules on Treg cells from Tln1^fl/fl or Tln1^fl/flFoxp3^Cre mice; displayed cells gated on CD4⁺Foxp3⁺ cells; n=6. (F) Suppression by sorted YFP⁺ Treg cells from Foxp3^Cre or Tln1^fl/flFoxp3^Cre mice at decreasing T_conv:T_reg cell ratios, measured at 72 hours. (G) Percentages of splenic Treg CD4⁺YFP⁺ cells expressing IFNγ, IL-17A and TNFα; n=6. Data are mean ± SEM and representative of at least 2 independent experiments. *, P < 0.05; **, P <0.01, *** P <0.005, unpaired Student’s t-test.