Figure 3. Expression of mutant talin(L325R) in Treg cells results in lethal systemic inflammation.

(A and B) Morphology (A) or organs (B), spleen, lymph nodes (aortic, brachial, mesenteric, inguinal), and thymi) of male Tln1^wt/flFoxp3^Cre or Tln1^L325R/flFoxp3^Cre mice. (C) Body weight of Tln1^wt/flFoxp3^Cre or Tln1^L325R/flFoxp3^Cre mice at 8 weeks of age; n=10. (D) Survival of Tln1^wt/flFoxp3^Cre or Tln1^L325R/flFoxp3^Cre; n=5. (E) Percentages of splenic CD4⁺and CD8⁺ T cells; n=6. (F) Expression of activation markers CD44 and CD62L from splenic CD4⁺and CD8⁺ T cells; n=6. (G) Expression of proliferation marker Ki-67 from splenic CD4⁺and CD8⁺ T cells; n=3. Data are mean ± SEM and representative of at least 2 independent experiments. *, P < 0.05; **, P <0.01, *** P <0.005, unpaired Student’s t-test.