Figure 4. Integrin activation is critical for T_reg cell homeostasis and function.

(A–B) Percentage (A) and by number (B) of splenic CD4⁺ T cells from Tln1^wt/flFoxp3^Cre or Tln1^L325R/flFoxp3^Cre mice expressing intracellular Foxp3; n=6. (C) Foxp3⁺ cells as a percentage of CD4⁺ T cells in various tissues (blood, thymus, liver, lung, and lymph node) from Tln1^wt/flFoxp3^Cre or Tln1^L325R/flFoxp3^Cre mice; n=6. Mice were perfused prior to isolation of cells from liver and lung. (D) MFI of Foxp3 from CD4⁺Foxp3⁺ Treg cells; n=6. (E) Expression of putative suppressor molecules on Treg cells from Tln1^wt/flFoxp3^Cre or Tln1^L325R/flFoxp3^Cre mice; displayed cells gated on CD4⁺Foxp3⁺; n=4. (F) Suppression by sorted YFP⁺ Treg cells from Tln^wt/flFoxp3^Cre and Tln1^L325R/flFoxp3^Cre mice at decreasing Tconv:Treg cell ratios, measured at 72 hours. Data are mean ± SEM and representative of at least 2 independent experiments. *, P < 0.05; **, P <0.01, *** P <0.005, unpaired Student’s t-test.