Figure 5. Talin controls the suppressive function of peripheral Treg cells.

(A) Percentages of talin-deficient Foxp3⁺ and YFP⁺ Treg cells from Tln1^wt/flFoxp3^Cre/wt or Tln1^fl/flFoxp3^Cre/wt mice, displayed cells gated on CD4⁺ events; n=7. (B) Percentages of splenic CD4⁺ (top) and CD8⁺ (bottom) T cells expressing CD44 and/or CD62L from female Tln1^wt/wtFoxp3^Cre/wt and Tln1^fl/flFoxp3^Cre/wt heterozygous mice; n=4. (C) Absolute number of Foxp3⁺YFP⁺ cells isolated from Tln1^wt/wtFoxp3^Cre/wt and Tln1^fl/flFoxp3^Cre/wt heterozygous mice; n=6. (D) Foxp3 MFI of Foxp3⁺YFP⁺ Treg cells from Tln1^wt/wtFoxp3^Cre/wt and Tln1^fl/flFoxp3^Cre/wt heterozygous mice, normalized to the Foxp3 MFI of Foxp3⁺YFP⁻ wild-type Treg cells from each individual animal; n=22. (E) Suppression by sorted YFP⁺ Treg cells from Tln1^wt/wtFoxp3^Cre/wt or Tln1^fl/flFoxp3^Cre/wt mice at decreasing Tconv:Treg cell ratios, measured at 72 hours. Expression (F) and MFI (G) of putative suppressive molecules by splenic Treg cells from Tln1^wt/wtFoxp3^Cre/wt or Tln1^fl/flFoxp3^Cre/wt mice; displayed cells gated on CD4⁺Foxp3⁺YFP⁺ cells; n=5. (H and I) Percentage (H) and absolute number (I) of eTreg (CD62L^loCD44^hi) and cTreg (CD62L^hiCD44^lo) in Tln1^fl/flFoxp3^Cre/wt and control mice; n=5. Data are mean ± SEM and representative of at least 2 independent experiments. *, P < 0.05; **, P <0.01, *** P <0.005, unpaired Student’s t-test.