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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Dev Biol. 2018 Apr 26;440(1):13–21. doi: 10.1016/j.ydbio.2018.04.020

Figure 1. Uniform activation of Rosa-Wnt1 ligand in NPCs of isolated metanephric mesenchymes promotes renewal.

Figure 1

Wildtype (A, B, D, E, G, I) or Six2-Cre; Rosa-Wnt1 (C, F, H, J) kidneys (A, D, G, I) or isolated metanephric mesenchyme (MM) (B, C, E, F, H, J) were dissected at E11.5 and cultured for 48 (A–F) or 96 hours (G–J). Tissues were fixed and hybridized with antisense probes to the Wnt9b renewal target gene, Cited1 (A–C) or the Wnt9b differentiation target gene, C1qdc2 (D–F). Immunofluorescence (IF) staining with antibodies to the NPC marker Six2, epithelial tubule marker E-cadherin and the UB marker pan-cytokeratin (green, red, white respectively in G, H) or Cited1, Six2, Pan-Cytokeratin (green, red, white respectively in I, J). All IF sections were counterstained with Dapi. Scale bars are 200uM for (A–F) and 100uM for (G–J).