Skip to main content
. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Dev Biol. 2018 Apr 26;440(1):13–21. doi: 10.1016/j.ydbio.2018.04.020

Figure 2. Failure of Six2-Cre;Rosa-Wnt1 metanephric mesenchymes to differentiate is not due to lack of UB-derived signals or secretion of functional Wnt protein.

Figure 2

Hematoxylin and Eosin staining (A–D), in-situ hybridization (E–H) or IF staining (I–O) of sections of E15.5 wildtype (A, E, I, M), Wnt9b−/− (B, F, J, N), Six2-Cre;Rosa-Wnt1 (C, G, K),Six2-Cre;Rosa-Wnt1;Wnt9b−/−(D,H,L) or Six2-CreERT2;Rosa-YFP;Rosa-Wnt1;Wnt9b−/−(O) kidneys. Tissues were hybridized with anti-sense probes for the Wnt9b differentiation target gene, C1qdc2 (E–H). Antibodies are to the NPC marker Six2 (gray), the proximal tubule marker LTL (green) and the UB marker pan-cytokeratin (red) in I–L or to GFP (green), renewal target Cited1 (red) and pan-cytokeratin (grey) in M–O. All IF sections were counterstained with DAPI. Scale bars are 50uM for (A–H),100uM for (I–L) or 25uM for (M–O).