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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Dev Biol. 2018 Apr 26;440(1):13–21. doi: 10.1016/j.ydbio.2018.04.020

Figure 6. Response to Wnt transgenes can be interchanged by altering ligand or beta-catenin levels.

Figure 6

Wildtype intact kidneys (A, H), wildtype isolated MM (B, I), Six2-Cre;CAG-Wnt9b isolated MM (C–E and J–L) or Six2-Cre;Rosa-Wnt1 isolated MM (F–G, M–N) were analyzed by whole mount in-situ hybridization with probes to Wnt9b renewal target gene, Cited1 (A–G) or Wnt9b differentiation target gene, C1qdc2 (H–N). Analysis was performed after 48 hours of culture in media supplemented with DMSO (A–C, F, H–J, M), a low dose (500nM) of the Porcupine inhibitor IWP2 (D, K), a low dose (1uM) of the tankyrase inhibitor IWR1 (E, L) or low dose (10mM) of LiCl (G, N). Scale bars are 200uM.