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. 2018 Apr 16;80(5):736–740. doi: 10.1292/jvms.17-0236

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©2018 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Effect of BSFL feeding on spleen cell proliferation in broiler chicks. Spleen cells were co-incubated with or without mitogen (ConA). Spleen cell proliferation was then determined as OD_540nm value of colored material after incubation with MTT. Spleen cell proliferation in BSFL-fed group was significantly enhanced compared to that of the control group (*, P<0.05; **, P<0.01). Proliferation of unstimulated cells did not show difference between groups. For each group, data are presented as mean OD_540nm ± SD (n=10).