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. 2018 Mar 16;80(5):790–797. doi: 10.1292/jvms.17-0657

Table 2. Fecal shedding of virus, clinical signs and immunohistochemistry findings after inoculation of pigs with porcine RVA strain HN03.

Pig status, pig no. Fecal shedding, PCR titer a), by hpi
Onset of clinical signs, hpi Hpi at euthanasia (after onset) Antigen detection by IHC
0 12 24 36 48 60 72 Duodenum Proximal jejunum Mid-jejunum Distal jejunum Ileum
HN03-inoculated
7 ND ND ND ND ND None 12.0
6 0 ND ND ND ND ND 16.0 18.0 (2) +++ ++++ ++++ +++
4 0 −3 ND ND ND ND 17.5 27.5 (10) + + +++ +
2 0 −3 −3 ND ND ND 22.5 42.5 (20) + ++ ++++ ++ +
10 0 −4 −3 ND ND ND 15.0 45.0 (30) + +++ ++++ ++ +
1 0 −3 −2 −2 −2 ND 22.5 62.5 (40) ++ ++ ++ ++ +
3 0 −3 −3 −2 −1 ND 17.5 67.5 (50) + + + +
9 0 −4 −3 −2 −2 −2 15.0 75.0 (60) + +++ + +

Negative control
5 ND ND ND ND None NA
11 None NA

−, negative; a) dilution 10n; hpi, hour post-inoculation; ND, not determined; NA, not applicable.