Figure 2.

Effect of paeoniflorin (PF) on L929 cell proliferation. A, According to the OD value, we calculated cell viability rate: cell viability rate = 1 − (OD490_test − OD490_blank)/(OD490_control − OD490_blank) × 100%. Graphpad Prism 7.0 was employed to calculate IC50 for further experiment. B, The cytotoxicity of PF on L929 cells. L929 cells were treated with increasing concentrations of PF (from 0.813 to 52 μg·mL⁻¹ for 24 hours, the cell viability was then determined by sulforhodamine B (SRB). C, Dose-dependent inhibition changes of L929 cells with PF incubation of 24 hours (10 ng·mL⁻¹ tumor necrosis factor α [TNF-α] as positive control). Compared to TNF-α (10 ng·mL⁻¹) group, *P < .05, **P < .01 (n = 3).